Continuing in the saga of "what do sequencing errors do to our de Bruijn graph density measure" (read the first post here), I have some new results.
The conclusion of the first post was that on random (non-real) genomes, both with and without repeats, we see that de Bruijn graph …
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Are our reviewers correct or incorrect?
About two months ago we got back reviews for our assembly artifacts paper, in which we showed that there was a strong 3' bias in the reads towards higher graph connectivity. Since shotgun sequencing is supposed to be random, we asserted that this 3' …
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The tech community is messed up in da head, yo.
Several times since Steve Holden's I'm Sorry post I've written long blog posts about my own views on codes of conduct and professional behavior, including the views informed by some of my own extraordinarily embarrassing transgressions. I never felt that …
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A week or two ago, I posted a crazy idea about crowdsourcing a bioinformatics analysis pipeline. I may still try to do that. But in the meantime, here's another crazy idea.
First, some background.
I'm writing this as I fly back from …
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Description
Random algorithms and probabilistic data structures are algorithmically efficient and can provide shockingly good practical results. I will give a practical introduction, with live demos and bad jokes, to this fascinating algorithmic niche. I will conclude with some discussions of how our group has applied this to …
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I'm worried about our current mRNAseq analysis strategies.
I recently posted a draft paper of ours to arXiv entitled RNA-Seq Mapping Errors When Using Incomplete Reference Transcriptomes of Vertebrates; the link is to the Haldane's Sieve discussion of the paper. Graham Coop said I should write something quick, and so …
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Over the last few weeks, I've been on a bit of a Cory Doctorow kick. I started by reading Homeland, a sequel to the excellent Little Brother; these two very important books about anti-terror-enabled government suppression of liberty and free speech are very well written and extremely timely. I then …
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