I'm worried about our current mRNAseq analysis strategies.
I recently posted a draft paper of ours to arXiv entitled RNA-Seq Mapping Errors When Using Incomplete Reference Transcriptomes of Vertebrates; the link is to the Haldane's Sieve discussion of the paper. Graham Coop said I should write something quick, and so …
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Note: this is the general part of the submitted review; I left out the things that I expect might change if revisions are made.
Also see Thoughts on the Assemblathon 2 paper.
Re the Assemblathon 2 paper <http://arxiv.org/abs/1301.5406>,
Bradnam et …
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(Also see Assemblathon 2 review, round 1, parts thereof)
I just finished reviewing the Assemblathon 2 paper, in which many of the extant de novo genome assembly pipelines were evaluated against three different organismal data sets. (I'll post the review when I can.) Good paper.
To me, the biggest outcome …
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We just posted yet another pre-submission paper to arXiv.org:
Assembling large, complex environmental metagenomes
Authors: Adina Chuang Howe, Janet Jansson, Stephanie A. Malfatti, Susannah Tringe, James M. Tiedje, and C. Titus Brown
Abstract:
The large volumes of sequencing data required to deeply sample …read more
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We just posted another pre-submission paper to arXiv.org:
Illumina Sequencing Artifacts Revealed by Connectivity Analysis of Metagenomic Datasets
Authors: Adina Chuang Howe, Jason Pell, Rosangela Canino-Koning, Rachel Mackelprang, Susannah Tringe, Janet Jansson, James M. Tiedje, and C. Titus Brown
Abstract:
Sequencing errors and …read more
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This post can be referenced and cited at the following DOI: http://dx.doi.org/10.6084/m9.figshare.98198.
For a few months, the Trinity list was awash with discussions about how to use digital normalization to lower the memory and compute requirements for mRNASeq assembly. At some point …
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I gave a talk last Wednesday at U. Michigan in the DCMB program where I included a slide estimating how much DNA sequencing (in base pairs) was needed for good de novo assembly of sequences from various biological environments or problems. The slide was there to motivate the challenges of …
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